Cleared leaf images currently displayed by ClearedLeavesDB are of specimens cleared and stained under the direction of Jack A. Wolfe, U. S. Geological Survey.

The collection contains over 14,000 leaves. The largest number of specimens in NCLC-W (7,460) were obtained from the University of California Herbarium at University of California Berkeley. Other contributing herbaria providing large numbers of specimens include the California Academy of Sciences (2,375), Arnold Arboretum, Harvard University (1,294), Missouri Botanical Garden (1,184), New York Botanical Garden (507), Field Museum of Natural History (388) and the National Herbarium of the Smithsonian Institution (172). When available we have provided the herbarium code and number for the sheet from which the cleared leaf was removed, and this number should be cited whenever possible.

Slides were photographed by Dane Miller, Erika Gonzalez, Ian Tom, Stephanie Bailey, and many volunteers at the Smithsonian Institution.